What this calculator does
It calculates concentration from peptide mass and solvent volume, then converts a chosen aliquot volume into an amount of material. It does not recommend protocols, administration, human use, veterinary use or therapeutic quantities.
Concentration calculator
Concentration—
Amount per aliquot—
Total vial volume—
Formula: concentration = mg ÷ mL. Aliquot amount = concentration × aliquot volume. Verify all calculations against your laboratory protocol.
Inputs to document in a research notebook
Vial identityProduct name, strength, batch/lot number and date opened.
SolventSolvent identity, volume added and any protocol-specific handling notes.
Final concentrationRecord mg/mL and µg/µL where helpful to reduce transcription errors.
Aliquot planLabel tubes with concentration, batch code, date and storage condition.
Common lab-math mistakes
- Mixing up mL and µL.
- Forgetting that 1 mg equals 1000 µg.
- Recording concentration but not the batch code.
- Using an aliquot after its internal protocol window.